Review

human kidney tubular hk2 cell line (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC human kidney tubular hk2 cell line
Human Kidney Tubular Hk2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hk2+human+kidney+2+cells/HK-2/pm42014688-221-0-10
Average 99 stars, based on 4540 article reviews

human kidney tubular hk2 cell line - by Bioz Stars, 2026-09

99/100 stars

Images

other:

Article Title: miR-4537 curtails ferroptosis by targeting MIOX in renal cell carcinoma.
Article Snippet: Ferroptosis, an iron-dependent mode of cell death, has gained prominence for its critical role in the advancement of various cancers, notably clear cell renal carcinoma (ccRCC).. The intricacies of ferroptosis’s involvement in ccRCC, however, remain largely undefined.. This study aimed to dissect the contribution of ferroptosis to ccRCC by examining differentially expressed genes (DEGs) identified within the TCGA ccRCC database and ferroptosis driver genes catalogued in the FerrDb database (dedicates to ferroptosis regulators and ferroptosis-disease associations).

Article Title: miR-4537 curtails ferroptosis by targeting MIOX in renal cell carcinoma
Article Snippet: Cell lines specific to ccRCC—ACHN and 786-O—as well as HK2 (human kidney −2) cells and HKC (human kidney cell, proximal tubule cell) were acquired from the National Collection of Authenticated Cell Cultures Center and the American Type Culture Collection.

Modification:

Article Title: Dual-Ligand Targeted Nanoparticles Enhance In Vitro Delivery and Efficacy of Investigational Drug Candidate GAT211.
Article Snippet: We present a dual-ligand polymeric nanoparticle system engineered via coassembly of two distinct ligand-functionalized polymers, one conjugated with gambogic acid (GA) as a targeting ligand, and the other conjugated with naringenin (NAR), which functions dually as a therapeutic agent and targeting ligand.. This design enables simultaneous and synergistic targeting of transferrin receptors (TfR) and folate receptors (FR).. In vitro studies using fluorescently labeled nanoparticles revealed significantly enhanced cellular uptake and receptor colocalization for dual-ligand nanoparticles compared to their corresponding singleligand or nontargeted controls.

Image Search Results

Klotho expression is not significantly regulated by aldosterone in MDCK, NRK-52E, and HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 4), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 5) cells treated with or without aldosterone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test. A.u., arbitrary units.

Journal: Biomolecules

Article Title: Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell Lines

doi: 10.3390/biom15111509

Figure Lengend Snippet: Klotho expression is not significantly regulated by aldosterone in MDCK, NRK-52E, and HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 4), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 5) cells treated with or without aldosterone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test. A.u., arbitrary units.

Article Snippet: Human kidney 2 (HK2) cells (CRL-2190, ATCC) were cultured at 5% CO 2 and 37 °C in DMEM with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. hTERT-immortalized primary renal proximal tubule epithelial cells (RPTEC/TERT1; CRL-4031, ATCC) were cultured under standard cell culture conditions in DMEM/F-12 with 2.5 mM L-glutamine and 15 mM HEPES (Gibco) supplemented with the hTERT immortalized RPTEC growth kit (ATCC-ACS-4007, ATCC), G418 solution (G418-RO, Sigma-Aldrich, Schnelldorf, Germany), 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Expressing, Gene Expression

Klotho expression is upregulated by spironolactone in MDCK and NRK-52E cells but downregulated in HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 5) cells treated with or without spironolactone for 24 h. Representative Western blot (left panel) and densitometric analysis of Klotho protein expression (right panel, n = 3) in HK2 cells treated with or without spironolactone (S) for 24 h ( D ). Note the Klotho band in the range of 120 kDa. The original Western blot images are shown in . One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test and two-tailed paired t -test; * p < 0.05, *** p < 0.001. A.u., arbitrary units.

Journal: Biomolecules

Article Title: Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell Lines

doi: 10.3390/biom15111509

Figure Lengend Snippet: Klotho expression is upregulated by spironolactone in MDCK and NRK-52E cells but downregulated in HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 5) cells treated with or without spironolactone for 24 h. Representative Western blot (left panel) and densitometric analysis of Klotho protein expression (right panel, n = 3) in HK2 cells treated with or without spironolactone (S) for 24 h ( D ). Note the Klotho band in the range of 120 kDa. The original Western blot images are shown in . One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test and two-tailed paired t -test; * p < 0.05, *** p < 0.001. A.u., arbitrary units.

Techniques: Expressing, Gene Expression, Western Blot, Two Tailed Test

Klotho expression is not significantly regulated by eplerenone in MDCK, NRK-52E, and HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 4) cells treated with or without eplerenone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Šidák’s multiple comparisons test. A.u., arbitrary units.

Journal: Biomolecules

Article Title: Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell Lines

doi: 10.3390/biom15111509

Figure Lengend Snippet: Klotho expression is not significantly regulated by eplerenone in MDCK, NRK-52E, and HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 4) cells treated with or without eplerenone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Šidák’s multiple comparisons test. A.u., arbitrary units.

Techniques: Expressing, Gene Expression

Klotho expression is downregulated by finerenone in MDCK and NRK-52E cells but not in HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 3) cells treated with or without finerenone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons test; ** p < 0.01. A.u., arbitrary units.

Journal: Biomolecules

Article Title: Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell Lines

doi: 10.3390/biom15111509

Figure Lengend Snippet: Klotho expression is downregulated by finerenone in MDCK and NRK-52E cells but not in HK2 cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A ), n = 5), NRK-52E (( B ), n = 5), and HK2 (( C ), n = 3) cells treated with or without finerenone for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons test; ** p < 0.01. A.u., arbitrary units.

Techniques: Expressing, Gene Expression

Testosterone and progesterone moderately increased Klotho expression in MDCK and NRK-52E cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A , D ), n = 5), NRK-52E (( B ), n = 7; ( E ), n = 6), and HK2 (( C , F ), n = 3) cells treated with or without testosterone ( A – C ) or progesterone ( D – F ) for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test; * p < 0.05, ** p < 0.01. A.u., arbitrary units.

Journal: Biomolecules

Article Title: Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell Lines

doi: 10.3390/biom15111509

Figure Lengend Snippet: Testosterone and progesterone moderately increased Klotho expression in MDCK and NRK-52E cells. Arithmetic means ± SEM of rel. Klotho gene expression in MDCK (( A , D ), n = 5), NRK-52E (( B ), n = 7; ( E ), n = 6), and HK2 (( C , F ), n = 3) cells treated with or without testosterone ( A – C ) or progesterone ( D – F ) for 24 h. One-way ANOVA followed by Dunnett’s multiple comparisons or Friedman test; * p < 0.05, ** p < 0.01. A.u., arbitrary units.

Techniques: Expressing, Gene Expression

Effect of M. japonicus leaf extract on erastin-induced cell death. (A) HK2 cells were treated with the indicated concentrations of erastin for 24 ‍h. (B) Cells were treated with erastin (0.5 ‍μM) and the indicated concentrations of M. japonicus leaf extract for 24 ‍h. (C) Cells were treated with the indicated concentrations of M. japonicus leaf extract for 24 ‍h. After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SD ( n = 5–6). ** p <0.01 vs untreated cells, ## p <0.01 vs erastin alone.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Effect of M. japonicus leaf extract on erastin-induced cell death. (A) HK2 cells were treated with the indicated concentrations of erastin for 24 ‍h. (B) Cells were treated with erastin (0.5 ‍μM) and the indicated concentrations of M. japonicus leaf extract for 24 ‍h. (C) Cells were treated with the indicated concentrations of M. japonicus leaf extract for 24 ‍h. After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SD ( n = 5–6). ** p <0.01 vs untreated cells, ## p <0.01 vs erastin alone.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: MTT Assay

Erastin increases intracellular Fe 2+ content, ROS production, and lipid peroxidation. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 3 ‍h (A) or 24 ‍h (B, C) and then incubated with (A) FerroOrange (1 ‍μM), (B) DHE (10 ‍μM), or (C) BODIPY 581/591 C11 (10 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope. Scale bar = 100 ‍μm.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Erastin increases intracellular Fe 2+ content, ROS production, and lipid peroxidation. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 3 ‍h (A) or 24 ‍h (B, C) and then incubated with (A) FerroOrange (1 ‍μM), (B) DHE (10 ‍μM), or (C) BODIPY 581/591 C11 (10 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope. Scale bar = 100 ‍μm.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Incubation, Fluorescence, Microscopy

Endocytosis inhibitors prevent erastin-induced intracellular Fe 2+ content and cell death. HK2 cells were pretreated with chlorpromazine (Cpz; 5 ‍μM), MitMab (Mit; 1 ‍μM), or ambroxol (Ax; 100 ‍μM) for 30 ‍min, followed by treatment with erastin (0.5 ‍μM) for 24 ‍h (A) or 3 ‍h (B). (A) After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SE ( n = 5). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone. (B) After treatment, cells were incubated with FerroOrange (1 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope. Scale bar = 50 ‍μm.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Endocytosis inhibitors prevent erastin-induced intracellular Fe 2+ content and cell death. HK2 cells were pretreated with chlorpromazine (Cpz; 5 ‍μM), MitMab (Mit; 1 ‍μM), or ambroxol (Ax; 100 ‍μM) for 30 ‍min, followed by treatment with erastin (0.5 ‍μM) for 24 ‍h (A) or 3 ‍h (B). (A) After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SE ( n = 5). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone. (B) After treatment, cells were incubated with FerroOrange (1 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope. Scale bar = 50 ‍μm.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: MTT Assay, Incubation, Fluorescence, Microscopy

Intracellular CD71 distribution. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 2 ‍h. After treatment, the cells were fixed, and immunocytochemistry was performed using antibodies against CD71 (green) and GM130 (red). The nuclei were stained with Hoechst 33342 (blue). Scale bar = 10 ‍μm.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Intracellular CD71 distribution. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 2 ‍h. After treatment, the cells were fixed, and immunocytochemistry was performed using antibodies against CD71 (green) and GM130 (red). The nuclei were stained with Hoechst 33342 (blue). Scale bar = 10 ‍μm.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Immunocytochemistry, Staining

M. japonicus leaf extract does not influence intracellular GSH levels or ferroptosis-related protein expression. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 24 ‍h. After treatment, (A) GSH levels were measured and (B) Western blotting was performed. The results are shown as the mean ± SE ( n = 3). ** p <0.01 vs vehicle. (C) Cells were treated with the indicated concentrations of RSL3 (1 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 24 ‍h. After treatment, cell injury was evaluated using an LDH assay. The results are shown as the mean ± SD ( n = 5–6). ** p <0.01 vs untreated cells.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: M. japonicus leaf extract does not influence intracellular GSH levels or ferroptosis-related protein expression. HK2 cells were treated with erastin (0.5 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 24 ‍h. After treatment, (A) GSH levels were measured and (B) Western blotting was performed. The results are shown as the mean ± SE ( n = 3). ** p <0.01 vs vehicle. (C) Cells were treated with the indicated concentrations of RSL3 (1 ‍μM) and M. japonicus leaf extract (20 ‍μg/ml) for 24 ‍h. After treatment, cell injury was evaluated using an LDH assay. The results are shown as the mean ± SD ( n = 5–6). ** p <0.01 vs untreated cells.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Expressing, Western Blot, Lactate Dehydrogenase Assay

Effect of several components in M. japonicus leaf extract on erastin-induced cell death. HK2 cells were treated with erastin (0.5 ‍μM) and the indicated concentrations of corilagin, geraniin, ellagic acid, gallic acid, rutin, mallotunic acid, or mallotusinic acid for 24 ‍h. After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SD ( n = 5). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Effect of several components in M. japonicus leaf extract on erastin-induced cell death. HK2 cells were treated with erastin (0.5 ‍μM) and the indicated concentrations of corilagin, geraniin, ellagic acid, gallic acid, rutin, mallotunic acid, or mallotusinic acid for 24 ‍h. After treatment, cell viability was measured using an MTT assay. The results are shown as the mean ± SD ( n = 5). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: MTT Assay

Effect of several components in M. japonicus leaf extract on erastin-induced ferroptosis. HK2 cells were treated with erastin (0.5 ‍μM) and corilagin, geraniin, ellagic acid , mallotunic acid, mallotusinic acid (0.1 ‍μM), or all five compounds (0.1 ‍μM each) for 24 ‍h (A, B, D, E) or 3 ‍h (C). After treatment, cell injury was evaluated by an MTT assay (A) and an LDH assay (B). The results are shown as the mean ± SD ( n = 3). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone. After treatment, cells were incubated with (C) FerroOrange (1 ‍μM), (D) DHE (10 ‍μM), or (E) BODIPY 581/591 C11 (10 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope or All-in-One fluorescence microscope. Scale bar = 50 ‍μm.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells

doi: 10.3164/jcbn.25-42

Figure Lengend Snippet: Effect of several components in M. japonicus leaf extract on erastin-induced ferroptosis. HK2 cells were treated with erastin (0.5 ‍μM) and corilagin, geraniin, ellagic acid , mallotunic acid, mallotusinic acid (0.1 ‍μM), or all five compounds (0.1 ‍μM each) for 24 ‍h (A, B, D, E) or 3 ‍h (C). After treatment, cell injury was evaluated by an MTT assay (A) and an LDH assay (B). The results are shown as the mean ± SD ( n = 3). ** p <0.01 vs vehicle, ## p <0.01 vs erastin alone. After treatment, cells were incubated with (C) FerroOrange (1 ‍μM), (D) DHE (10 ‍μM), or (E) BODIPY 581/591 C11 (10 ‍μM) for 30 ‍min. Fluorescence was visualized using a confocal laser microscope or All-in-One fluorescence microscope. Scale bar = 50 ‍μm.

Article Snippet: Human kidney tubule HK2 cells (ATCC) were cultured in DMEM/Ham’s F-12 medium containing 10% (v/v) fetal calf serum (FCS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: MTT Assay, Lactate Dehydrogenase Assay, Incubation, Fluorescence, Microscopy

Review

human kidney tubular hk2 cell line (ATCC)

Structured Review

Images

Related Articles

other:

Modification:

Similar Products

Image Search Results